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Image Search Results
Journal: The American Journal of Pathology
Article Title: Collagen VI Deficiency Results in Structural Abnormalities in the Mouse Lung
doi: 10.1016/j.ajpath.2019.10.014
Figure Lengend Snippet: COL6 regulates lung epithelial cell monolayer density. A: Representative images of plasma membrane staining of fully confluent monolayers of 16HBE cells on COL6, Matrigel, and COL1 matrix coatings. B: Quantification of cell density (cells/field) from A. C: Z-scores representing predicted activity of elected canonical pathways significantly associated with differentially expressed genes in Col6a1−/− mouse lungs. D and E: Western blot analysis (D) and quantification of phosphorylated paxillin (pPax) relative to total paxillin (E). F: Quantification of the percentage change in cell density (cells/field) in cells treated with inhibitors of phosphatidylinositol 3-kinase (PI3K), Cdc42, RhoA, focal adhesion kinase (FAK), Rac1, and extracellular signal-regulated kinase (ERK) at steady state relative to untreated cells on COL6, COL1, and Matrigel. Data are expressed as means ± SD (B). n = 6 (B and F); n = 3 (E). *P < 0.05, **P < 0.01 versus Matrigel. Scale bar = 25 μm (A). MAPK, mitogen-activated protein kinase; PAK, p21 activated kinase; RhoGDI, rho GDP dissociation inhibitor.
Article Snippet: Tissue culture plates were coated with either COL6 from human placenta (009-001-108; Rockland, Limerick, PA),
Techniques: Staining, Activity Assay, Western Blot